On the Numerical Computation of Enzyme Kinetic Parameters

Authors

  • Stanko Dimitrov Sofia University “St. Kliment Ohridski”, Faculty of Mathematics and Informatics
  • Gergana Velikova University of Sofia, Faculty of Mathematics and Informatics
  • Venko Beschkov Institute of Chemical Engineering, Bulgarian Academy of Sciences
  • Svetoslav Markov Institute of Mathematics and Informatics, Bulgarian Academy of Sciences

DOI:

https://doi.org/10.11145/j.bmc.2015.02.201

Abstract

We consider the enzyme kinetic reaction scheme originally proposed by V. HenriВ  of single enzyme-substrate dynamics where two fractions of the enzyme - free and bound - are involved. Henri's scheme involves four concentrations and three rate constants and via the mass action law it is translated into a systemВ  of four ODE's. On two case studies we demonstrate how the rate constants can be computed whenever time course experimental data are available. The obtained results are compared with analogous results implied by classical Michaelis-Menten model. Our approach focuses on the uncertainties in the experimental data, as well as on the use of contemporary computational tools such as CAS Mathematica.

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Published

2015-02-16

Issue

Vol. 1 No. 2 (2014)

Section

Articles

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